Tthssb

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WebJun 1, 2005 · Here, we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in multiplex PCR of human Y-STR markers identification.The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction … Webdate, offering an attractive alternative to TaqSSB and TthSSB in molecular biology applications, especially with using high temperature e. g. polymerase chain reaction (PCR). Background Single-stranded DNA-binding (SSB) proteins play an essential role in allin vivo processes involving ssDNA. They interact with ssDNA and RNA, in an independent

Application of SSB-like protein from - ScienceDirect

WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse spontaneously along ssDNA over a wide range of salt concentrations (20-500mM NaCl), and that TthSSB diffusion can help transiently melt the DNA hairpin structures. WebThe N-terminal domain is located in the region from amino acid 1 to 123 and the C-terminal domain is located between amino acids 124 and 264 or 266 in TthSSB and TaqSSB, … how many cinemas in uk

ssDNA Binding Proteins NEB

WebNov 1, 2003 · Europe PMC is an archive of life sciences journal literature. WebAug 10, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the … WebJul 27, 2006 · TthSSB-255 could only enhance RCA reactions drastically, which showed weak ssDNA binding affinity. This characteristic of the protein would be critical for reduction of nonspecific products. As shown in Supplementary Figure SP2, TthSSB has a significant cluster consisting of acidic residues in its C-terminus although the role has not been … how many cinemas in australia

Identification and characterization of single-stranded-DNA-binding ...

TthSSB appears to be positioned near the center of the

WebMay 17, 2006 · We have overexpressed the native and mutant forms of TthSSB protein in Escherichia coli and purified them to homogeneity. In vitro, these proteins were found to … WebIn vitro, these proteins were found to bind specifically to single-stranded DNA. Addition of TthSSB mutant protein to RCA halved the elongation time required for phi29 DNA … how many cinemas in londonWebThermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA speciﬁcally over a temperature range of 4–80oC, and the wild-type protein could withstand incubation at 94oC for 2 min. Addition of TthSSB to PCR halved the elonga-tion time required for the DNA polymerases of high school musical bet on it

"WebDespite this, the ®delity of the Tth DNA polymerase in the presence of TthSSB in these assays was an order of magnitude lower than that obtained with the proof-reading … " - Tthssb

Tthssb

A highly thermostable, homodimeric single-stranded DNA-binding …

WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95°C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes. WebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over linear targets. Crown",

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Webfrom T.thermophilus strain HB8 (TthSSB) increases the efﬁ-*To whom correspondence should be addressed. Tel: +81 45 508 7224; Fax: +81 45 508 7364; Email: [email protected] The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors The Author 2006. Published by Oxford University Press. http://www.100md.com/html/DirDu/2006/07/27/12/18/65.htm

WebA fixed quantity (20 pmol) of 5h-end fluorescein-labelled oligonucleotides (dT) 30 , (dT) 60 , (dT) 70 or (dT) 76 was incubated with 0, 5, 20, 50 and 200 pmol of TthSSB in 20 µl … WebOct 1, 2002 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand. 44. PDF. View 1 excerpt, cites background;

WebFurthermore, TthSSB increases the fidelity of proofreading deficient Thermus thermophilus DNA polymerase (Perales et al., 2003). We tested the slippage properties of PabPolB, ... WebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately …

WebA fixed quantity (20 pmol) of 5h-end fluoresceinlabelled oligonucleotide (dT) 30 was incubated with 20 pmol of TaqSSB or TthSSB (#) in 20 µl reaction mixtures in standard …

WebNov 15, 2003 · The thermostability of TthSSB was also studied. To this end, TthSSB was pre‐incubated at 94°C for different times, cooled down to 70°C, and immediately subjected to bandshift assays. As shown in Figure 2 C, TthSSB could withstand up to 2 min incubation at 94°C, without any detectable loss of its ssDNA‐specific binding activity. how many cipro pillsWebOct 1, 2002 · TthSSB and TaqSSB monomer proteins contain 264 and 266 amino acids, respectively.The homology between these proteins is very high—82% identity and 90% … how many ciphers did the zodiac killer sendWebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … how many circle k stores in californiaWebAug 8, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand high school musical bilibiliWeb(A and B) Representative single-molecule time traces (A) and average cross-correlation curves (B), for (dT)65+hp+3 alone and TthSSB-bound (dT)65+hp+3, obtained at 500 mM … high school musical bg audio full movieWebMay 12, 2009 · Use of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500x. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over … how many cinnamon capsules per dayWebOct 1, 2002 · The nucleotide (nt) sequence revealed that T. thermophilus SSB (TthSSB) and T. aquaticus (TaqSSB) consist of 264 and 266 amino acids, respectively, and have a molecular weight of 29.87 and 30.03kDa, respectively. The homology between these protein, is very high-82% identity and 90% similarity. They are the largest known prokaryotic SSB … high school musical bet on it lyrics