Sh-sy5y lipofectamine 3000
HepG2, Huh7, PC3, SH-SY5Y, HEK293, MCF7, HL-60, and A549 cancer cell lines were obtained from American Type Culture Collection (ATCC). All cell lines were mycoplasma-free when periodically tested with Mycoplasmacheck (Eurofins Genomics) or MycoProbe (R&D). Cells were cultured in T-75 flasks … See more pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP10(15.0 kb), FC3-GFP (6.2 kb), SpCas9(BB)-2A-GFP-prickle (9.2 kb), pCAGGs-jmj1dc-IRES-GFP (13.3 kb), pUC19-no … See more Cells were electroporated using the NEON electroporation system (Invitrogen). Briefly, cells grown to 70–90% confluency were harvested and pelleted at 500 g for 5 … See more Liposomal transfection was done with lipofectamine 3000 according to the manufacturer’s instructions. All cells were plated in 24-well plates (110,000/well HepG2 … See more Transfection efficiency was measured after 24 h (unless stated differently) by flow cytometry (FACSNavios, Beckman Coulter, Navios Cytometry List Mode … See more WebApr 8, 2024 · The SH-SY5Y neuroblastoma cells were obtained from ATCC (#CRL-2266). Cells were cultured in DMEM with 10% FBS and 1X Penicillin-Streptomycin on 0.1% gelatin-coated plates, passaged with 0.05% ...
Sh-sy5y lipofectamine 3000
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WebLipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, ... SH-SY5Y: 1: … WebApr 12, 2024 · For differentiation of SH-SY5Y cells into neurons, cells were incubated for 48 h with media that contained 1% FBS and 100 μM all-trans retinoic acid (Thermo, #207341000). Lipofectamine™3000 transfection reagent (ThermoFischer, #3000-008) was used for transient expression experiments. Dorsal root ganglion ...
WebVectors carrying guides targeting Atg7 were transfected into L6 cells using Lipofectamine 3000. Transduced cells were selected using 1 μg/ml puromycin over ... Bandyopadhyay J. … WebMar 9, 2024 · Similar results were obtained using human neuroblastoma SH-SY5Y cells and primary neurons (Supplementary Figure S1), suggesting that the extract has a potent ...
WebEnter the email address you signed up with and we'll email you a reset link. WebJan 31, 2024 · (54)【発明の名称】ALSのバイオマーカーおよびALSの診断方法
WebSH-SY5Y cells in 6-well plates (40×10 4 cells/ml) were transfected with 4 µg of human SIRT3 cDNA using Lipofectamine 3000 reagent (Invitrogen). An empty pCMV6-Flag vector was …
WebApr 1, 2024 · Human SH-SY5Y cells were grown to 70%–80% confluence, starved for 24 h in serum-free medium and transfected with plasmid vector using Opti-MEM (Gibco) and Lipofectamine 3000 transfection reagent (Invitrogen, Carlsbad, CA, USA) as previously described according to the manufacturer's recommendations. 18. Isolation of mitochondria dave chappelle house of blues houston ticketsWebsh-sy5y Cells. Organism Type: Human: A subline of the neuroblastoma SK-N-SH line derived from a metastatic bone tumor. ... Recommended Transfection Reagents. Plasmid DNA … dave chappelle house of dragon skithttp://www.protocol-online.org/biology-forums-2/transfection.html black and gold night dressWebFriedreich’s ataxia (FA) is an autosomal reccessive neurodegenerative disorder, which outcome mostly from reduced expression of and withochondrial protein frataxin. FA has … black and gold new orleans saints storeWebMar 4, 2024 · 1.1.2酶及主要试剂 总RNA提取试剂Trizol、转染试剂Lipofectamine 3000购自Invitrogen公司 ... 在聚丙烯管里准备Lipectamine 3000和DNA复合物：在聚丙烯管中加入0.5 mL预热的Opti-MEM培养基，加入miR-195模拟物100 nM和 ... Survivin-siRNA重组质粒对人神经母细胞瘤细胞SH-SY5Y ... black and gold new era hatWebConditions were set up according to the manufacturer’s recommendations both for Lipofectamine ® 3000 and for jetOPTIMUS ... "I tried both jetOPTIMUS® and jetPRIME® … black and gold nightstandsWebProgressive degeneration of dopaminergic neurons characterizes Parkinson’s disease (PD). This neuronal loss occurs through diverse mechanisms, including a form of programmed cell death dependent on... black and gold new years nails