Hoechst 33342 apoptosis
NettetThe adenosine-induced apoptosis was determined by Hoechst 33342 staining and flow cytometry. The effect of adenosine on the intracellular reactive oxygen species (ROS) … NettetHoechst 33342 induces apoptosis in BC3H-1 myocytes and hepatoma cells. Objective: To determine if Hoechst 33342 or Hoechst 33258 induces apoptosis in human …
Hoechst 33342 apoptosis
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Nettet1. sep. 1994 · Abstract A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with … Nettet25. des. 2008 · Hoechst 33342, but not Hoechst 33258, induced apoptosis in the HL-60 cells in a time- and dose-dependent manner. Endogenous nuclear topoisomerase I activity in HL-60 cells was inhibited by ...
Nettet16. sep. 2024 · A staining dye solution was prepared by adding 1 µL of calcein AM (4 mM stock from Invitrogen), 1 µL Hoechst 33342, and 10 µL CellEvent ® Caspase-3/7 into 8 mL of staining buffer. The staining dye solution was dispensed at 1 µL per microwell in the microwell chips which was then combined with the micropillar chip containing the cells … NettetHoechst 33342-induced apoptosis is associated with disruption of TATA box binding protein/TATA box complexes, replication protein A/single-stranded DNA complexes, topoisomerase I/DNA cleavable complexes and with an increased intracellular concentration of E2F-1 transcription factor and nitric oxide concentration.
Nettet您好！欢迎来到炼石商城 请登录 注册 我的订单; 我的炼石 NettetHoechst 33342, 10 mg/mL in Water: 40046: 561.93: 10 mL: 350/461 nm: Yellow solution: Hoechst 33342, trihydrochloride trihydrate: 40047: 615.98: 100 mg: 350/461 nm: …
Nettet10. okt. 2024 · Hoechst 33342 dye was used to assess morphological changes of apoptosis. Expression levels of Akt, FoxO3a, GSK-3β, and apoptosis- and cell cycle-associated proteins were detected by Western blotting. Tube formation assay was used to test the angiogenesis role of conditioned medium from MSCs in vitro. The cytokine …
NettetThe adenosine-induced apoptosis was determined by Hoechst 33342 staining and flow cytometry. The effect of adenosine on the intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) was assessed according to the DCFH-DA and JC-1 methods, respectively. dr mattar cancer center of kansasNettet1. des. 2010 · Little is currently known about possible toxicity of fluorescent DNA dyes under CTLM conditions. In this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in the absence of imaging at high dye concentrations, phototoxicity from … dr. matt and dr. mike\u0027s medical podcastNettet1. okt. 1999 · In BC3H-1 myocytes, Hoechst 33342–induced apoptosis is independent of de novo RNA and protein synthesis and p53 expression.29 However, lactate oxidation rate, a measure of mitochondrial function,35 is reduced in Hoechst 33342–treated BC3H-1 myocytes, suggesting that mitochondrial dysfunction36 also plays a role in this … cold medicine for hypothyroid patientsNettet18. jun. 2016 · Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the enhanced Bax/Bcl-2 ratio. What’s more, compound 1 also promoted the expression of the tumor suppressor p53 protein. dr matta townsvilleNettet18. feb. 2024 · For this experiment, the mechanism for which cell death ensues can be elucidated by the use of two different stains: Hoechst 33342 and propidium iodide. In the case of apoptotic cells with intact membranes, Hoechst 33342 is able to permeate through the membrane to stain the DNA of cells. cold medicine for infants and toddlersNettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM). dr matt aurora racine wiNettet29. apr. 2024 · Apoptosis is a physiological process that occurs commonly during the development of the preimplantation embryo. The present work examines the ability of apoptotic embryonic cells to express a... cold medicine for kittens